For example, if you have a sample which was stained for CD8 and CD4, you can automatically create 8 gates that representĪll combinations of expression of the cytokines (e.g., CD4+CD8+, CD4+CD8-, CD4-CD8-) using FlowJo's Boolean Combination option.
TIZEN STORE APPS TO GEAR S3 SERIES
What the Boolean expression is you can't include a Monocytes gate)-if you specify NOT Lymphocytes in the expression then you would get no cells! In this case, you probably want to add the Boolean gate at the sample level.īecause all parent gates are automatically part of the final gate (from FlowJo's standard hierarchical approach), they will not appear in the popup menu of possible subsets to include in the expression.įlowJo can also automatically create a series of Boolean gates that represent all combinations (plus and minus) of a set of gates. For example, if you add a Boolean expression to the Lymphocyte gate, then only lymphocytes can be part of the gate (no matter Note that the Boolean expression you specify is applied to the cells in the subset to which you are adding the Boolean gate. For example: Live AND (CD4 or CD8) AND NOTGammaDelta willĬreate a gate that includes cells in the predefined Live gate, GammaDelta gate, and either the CD4 or CD8 gates. Boolean expressions can be arbitrarily complex you can use parentheses to associate the expression properly. Otherwise, you would almost always want to use relative specification.īoolean gates are "live", meaning that they will reflect any changes you make to the gates on which they depend. So, use absolute specification if you need to move the boolean gates and they refer to gates that are at different levels. Want to do is to specify SubsetX AND SubsetY as relative, and Monos as absolute path-so that the Monos references would be correct no matter where in the heirarchy the Boolean gate is dragged. If you now dragged this gate to the LiveGate under SubsetĬ, then the references to Subset X AND Subset Y work just fine (because those exist at the relative level under Subset C-but it won't find the Monos gate because that one is now one more level up the hierarchy. In relative terms, this is specified as /Subset X AND /Subset Y AND NOT ///Monos - i.e., have to go up three levels to get to Monos.
Consider a gate that you want to add to Subset A which is something like Subset X AND Subset Y AND NOT Monos (i.e., you want events that are both in Subset X and Subset Y but With absolute reference, they would still refer to the ones under the Subset A gate-which is probably not what you wanted.īut there are times when you want absolute references.
TIZEN STORE APPS TO GEAR S3 SOFTWARE
If you add a boolean gate to Subset A which is Subset X OR Subset Y with relative paths, and then drag that boolean gate to Subset B, the boolean gate will now change to refer to the gates Subset X and Subset Y that are under the While the gear s3 is a nice piece of hardware, the software support is still poor and if it was possible to get other packages working on the s3 via the Tizen store (even if they are not visually aligned to the gears design), its still a far stretch better then no app at all lol.
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